I thought getting into the mechanism…even just the idea of a mechanism…would be useful. It turns out I posted this earlier in the year when we were first looking at enzymes. But, now that we are further along in the year and we are looking at the role of enolase in glycolysis, I thought I would repost it. This enzyme catalyzes the step right before pyruvate kinase, the second "pay-off" or substrate-level phosphorylation step.